一种针对人Ia样抗原的单克隆抗体（MoAb Q2/70）的血清学、功能及免疫化学特性分析

Serological, functional, and immunochemical characterization of a monoclonal antibody (MoAb Q2/70) to human Ia-like antigens.

作者信息

Quaranta V, Indiveri F, Glassy M C, Ng A, Russo C, Molinaro G A, Pellegrino M A, Ferrone S

出版信息

Hum Immunol. 1980 Oct;1(3):211-23. doi: 10.1016/0198-8859(80)90016-6.

DOI:10.1016/0198-8859(80)90016-6

Abstract

Serological and immunochemical studies showed that monoclonal antibody Q2/70 (MoAb Q2/70), produced by the hybridoma technique, is specific for human Ia-like antigens. This antibody recognizes an antigenic determinant which is different from those defining the serologic polymorphism of Ia-like antigens, and is expressed on subsets of human Ia-like molecules and on lymphoid cells from other species. MoAb Q2/70 inhibits unidirectional MLRs* between allogenic human lymphocytes, but not between murine and human lymphocytes. In ADCC* assays. MoAb Q2/70 mediates lysis of cultured human B lymphoid cells RPMI 4098, effected by murine splenocytes. The antibody is suitable to isolate immunologically functional B lymphocytes from human peripheral blood.

摘要

血清学和免疫化学研究表明，通过杂交瘤技术产生的单克隆抗体Q2/70（MoAb Q2/70）对人Ia样抗原具有特异性。该抗体识别一种抗原决定簇，它不同于那些定义Ia样抗原血清学多态性的抗原决定簇，并且在人Ia样分子的亚群以及来自其他物种的淋巴细胞上表达。MoAb Q2/70抑制同种异体人淋巴细胞之间的单向混合淋巴细胞反应（MLRs*），但不抑制小鼠和人淋巴细胞之间的反应。在抗体依赖的细胞介导的细胞毒性（ADCC*）试验中，MoAb Q2/70介导由小鼠脾细胞对培养的人B淋巴细胞RPMI 4098的裂解作用。该抗体适用于从人外周血中分离具有免疫功能的B淋巴细胞。

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