纤细裸藻叶绿体延伸因子Ts的纯化
Purification of Euglena gracilis chloroplast elongation factor Ts.
作者信息
Fox L, Brot N, Weissbach H
出版信息
J Biol Chem. 1981 Aug 10;256(15):7796-9.
Previous studies have demonstrated that the activity of Euglena gracilis chloroplast elongation factor Ts is regulated by light and the gene for this factor is nuclear coded (Fox, L., Erion, J., Tarnowski, J., Spremulli, L., Brot, N., and Weissbach, H., J. Biol. Chem. (1980) 255, 6018-6019). Chloroplast elongation factor Ts has been purified to near homogeneity from E. gracilis extracts prepared from light-grown cells. It is composed of a single polypeptide chain of approximately 62,000 daltons. It interacts stoichiometrically with Escherichia coli elongation factor Tu to form an elongation factor Tu.elongation factor Ts complex.
先前的研究表明，纤细裸藻叶绿体延伸因子Ts的活性受光调节，且该因子的基因由细胞核编码（福克斯，L.，埃里翁，J.，塔尔诺夫斯基，J.，斯普雷姆利，L.，布罗特，N.，和魏斯巴赫，H.，《生物化学杂志》（1980年）255卷，6018 - 6019页）。叶绿体延伸因子Ts已从由光照培养的细胞制备的纤细裸藻提取物中纯化至接近均一。它由一条约62,000道尔顿的单多肽链组成。它与大肠杆菌延伸因子Tu按化学计量相互作用形成延伸因子Tu - 延伸因子Ts复合物。
Zotero 插件