Black O, Murrill E, Fanska C
Res Commun Chem Pathol Pharmacol. 1981 Jul;33(1):103-18.
Because of the high incidence of pancreatic cancer in the United States and because of the correlation of pancreatic cancer to environmental exposure, we have undertaken experiments to measure the metabolism of the carcinogen 7,12-dimethylbenzanthracene in the pancreas of the Long-Evans male rat. This study examined the in vitro metabolism of the carcinogen and found the production of aqueous products in pancreas to be similar to that in liver, however, the pancreatic capability was not induced to greater metabolism by pretreatment with either phenobarbital or 3-methylcholanthrene. High pressure liquid chromatographic analysis of the in vitro products of pancreatic metabolism demonstrated a relatively greater abundance of 5,6-epoxy-7-hydroxymethyl-12-methylbenzanthracene than the liver and a relatively less abundance of 7-hydroxymethyl-12-methylbenzanthracene and 7-methyl-12-hydroxymethylbenzanthracene than the liver. Carcinogen levels were measured in pancreas, liver, bile and blood at 2, 5, 10, 16, 22 and 36 hours after injection.
由于胰腺癌在美国的高发病率以及胰腺癌与环境暴露的相关性，我们开展了实验来测定致癌物质7,12 - 二甲基苯并蒽在长 - 伊文斯雄性大鼠胰腺中的代谢情况。本研究检测了该致癌物质的体外代谢，发现胰腺中水性产物的生成与肝脏相似，然而，用苯巴比妥或3 - 甲基胆蒽预处理并未诱导胰腺产生更强的代谢能力。对胰腺代谢体外产物的高压液相色谱分析表明，与肝脏相比，5,6 - 环氧 - 7 - 羟甲基 - 12 - 甲基苯并蒽的含量相对更高，而7 - 羟甲基 - 12 - 甲基苯并蒽和7 - 甲基 - 12 - 羟甲基苯并蒽的含量相对更低。在注射后2、5、10、16、22和36小时测量胰腺、肝脏、胆汁和血液中的致癌物质水平。
