Tsukamura M, Mizuno S, Murata H
Tubercle. 1981 Mar;62(1):43-6. doi: 10.1016/0041-3879(81)90034-9.
Out of 97 strains of M. tuberculosis isolated from the sputum of the same number of patients, 11 were shown to consist of a mixed population of M. tuberculosis and a strain of the M. avium-M. intracellulare (MAI) complex. The most effective method for isolating strains of the MAI complex was to incubate the culture first at 42 degrees C for 3 weeks and then at 45 degrees C for 3 weeks. By this method, the growth of M. tuberculosis was suppressed, while the contaminant mycobacteria formed colonies.
从相同数量患者的痰液中分离出的97株结核分枝杆菌中,有11株被证明是结核分枝杆菌与鸟分枝杆菌-胞内分枝杆菌(MAI)复合群中的一个菌株的混合菌群。分离MAI复合群菌株的最有效方法是先将培养物在42℃下孵育3周,然后在45℃下孵育3周。通过这种方法,结核分枝杆菌的生长受到抑制,而污染的分枝杆菌形成菌落。