Barta O, Oyekan P P
Am J Vet Res. 1981 Mar;42(3):378-81.
Optimal conditions for assaying feline (cat) hemolytic complement (C) were determined. Effects of the following factors on C titers were tested: pH, ionic strength; calcium, magnesium, and ethylenediamine tetraacetate concentrations; time and temperature of incubation. Guinea pig erythrocytes sensitized with feline antibody were the most sensitive target cells for feline C. A buffer of pH 7.3, with an ionic strength of 0.06 and containing 0.3 mM CaCl2 and 1 mM MgCl2, was optimal for feline C assay. A concentration of 4 mM ethylenediamine tetraacetate in calcium- and magnesium-free buffer inhibited the C reactivity to 1/53rd of the original titer. Sera from clinically healthy cats contained 70 to 150 CH50 (50% hemolytic) U/ml when tested, using 1 x 10(8) sensitized guinea pig erythrocytes/ml.
确定了检测猫溶血补体（C）的最佳条件。测试了以下因素对C滴度的影响：pH值、离子强度、钙、镁和乙二胺四乙酸浓度、孵育时间和温度。用猫抗体致敏的豚鼠红细胞是猫C最敏感的靶细胞。pH值为7.3、离子强度为0.06且含有0.3 mM氯化钙和1 mM氯化镁的缓冲液最适合用于猫C检测。在无钙和无镁缓冲液中4 mM乙二胺四乙酸的浓度可将C反应性抑制至原始滴度的1/53。当使用每毫升1×10⁸个致敏豚鼠红细胞进行检测时，临床健康猫的血清含有70至150 CH50（50%溶血）单位/毫升。
