Canto J, Biberstein E L, Schulte T A, Behymer D
J Clin Microbiol. 1983 Mar;17(3):500-6. doi: 10.1128/jcm.17.3.500-506.1983.
The specificity and sensitivity of agglutination, complement fixation, and enzyme-linked immunosorbent assay (ELISA) procedures in the detection of antibodies to Haemophilus somnus was investigated. H. somnus rabbit immune sera were found to agglutinate Pasteurella multocida, Staphylococcus aureus, and Haemophilus agni and, in some instances, also Pasteurella haemolytica, Salmonella dublin, Streptococcus agalactiae, and Corynebacterium pyogenes. In complement fixation tests with saline extracts as antigens, only H. agni reacted with H. somnus antisera to any significant degree. In ELISA tests with sonicated or heat-extracted antigens, cross-reactions were seen with the two Pasteurella spp. and with H. agni. When whole cells and saline extracts were used as antigens in ELISAs, only H. agni showed any cross-reactivity. The greatest specificity in distinguishing homologous from heterologous reactions was achieved by ELISA with saline extracts as antigens. Escherichia coli and Brucella abortus antigens failed to react with H. somnus antibody in any of the tests. A rabbit serum containing antibody to bovine type isolates of P. multocida, P. haemolytica, S. aureus, S. agalactiae, S. dublin, C. pyogenes, and E. coli gave no positive reaction in ELISA tests with saline extract of H. somnus as antigen. It is concluded that such saline extract, which appears to consist largely of H. somnus common antigen, has the potential of being a useful diagnostic reagent in the study by ELISA of antibody response to H. somnus.
研究了凝集试验、补体结合试验和酶联免疫吸附测定（ELISA）检测睡眠嗜血杆菌抗体的特异性和敏感性。发现睡眠嗜血杆菌兔免疫血清能凝集多杀巴斯德菌、金黄色葡萄球菌和无乳嗜血杆菌，在某些情况下还能凝集溶血巴斯德菌、都柏林沙门菌、无乳链球菌和化脓棒状杆菌。在用盐水提取物作为抗原的补体结合试验中，只有无乳嗜血杆菌与睡眠嗜血杆菌抗血清有显著反应。在用超声处理或热提取抗原的ELISA试验中，观察到与两种巴斯德菌属以及无乳嗜血杆菌有交叉反应。当全细胞和盐水提取物用作ELISA的抗原时，只有无乳嗜血杆菌表现出任何交叉反应。以盐水提取物作为抗原的ELISA在区分同源反应和异源反应方面具有最高的特异性。大肠杆菌和流产布鲁氏菌抗原在任何试验中均未与睡眠嗜血杆菌抗体发生反应。一份含有针对多杀巴斯德菌、溶血巴斯德菌、金黄色葡萄球菌、无乳链球菌、都柏林沙门菌、化脓棒状杆菌和大肠杆菌牛型菌株抗体的兔血清，在以睡眠嗜血杆菌盐水提取物作为抗原的ELISA试验中未出现阳性反应。得出的结论是，这种似乎主要由睡眠嗜血杆菌共同抗原组成的盐水提取物，有可能成为ELISA研究睡眠嗜血杆菌抗体反应的有用诊断试剂。
