使用细胞化学切片生物测定法评估不同胃泌素肽的生物活性。

The biological activity of different gastrin peptides assessed using a cytochemical section bioassay.

作者信息

Askew A R, Napier B J

出版信息

Aust N Z J Surg. 1981 Aug;51(4):341-5. doi: 10.1111/j.1445-2197.1981.tb04960.x.

DOI:10.1111/j.1445-2197.1981.tb04960.x

Abstract

A cytochemical section bioassay has been used to assess the carbonic anhydrase activity, induced by the gastrin peptides G13, G17 and G34 (concentration 5.0-0.005 pg/ml), in guinea pig parietal cells. When the mean integrated density of the induced response x 100, was plotted against the concentration of the gastrin peptide used, a graded dose response and a positive linear correlation was found for G13 and G17. These responses were similar. G34 did not produce a linear dose response in the concentration range 5.0-0.005 pg/ml, but a higher overall carbonic anhydrase activity was observed than with G13 and G17.

摘要

已采用细胞化学切片生物测定法评估胃泌素肽G13、G17和G34（浓度为5.0 - 0.005 pg/ml）在豚鼠壁细胞中诱导产生的碳酸酐酶活性。当将诱导反应的平均积分密度乘以100后，针对所使用的胃泌素肽浓度作图时，发现G13和G17呈现出分级剂量反应和正线性相关性。这些反应相似。在5.0 - 0.005 pg/ml浓度范围内，G34未产生线性剂量反应，但观察到其总体碳酸酐酶活性高于G13和G17。