Lombardi R, Mannucci P M, Seghatchian M J, Garcia V V, Coppola R
Br J Haematol. 1981 Sep;49(1):61-8. doi: 10.1111/j.1365-2141.1981.tb07197.x.
Factor VIII-related antigen (VIIIR:Ag) was consistently higher than factor-VIII procoagulant activity (VIII:C) in 57 patients with clinical conditions characterized by acute-phase reactions. Two different methods for measuring VIII:C (one- and two-stage assays) and VIIIR:Ag (electroimmunodiffusion and immunoradiometric assay) gave concordant results in the majority of cases. In 43% of plasma samples, crossed immunoelectrophoresis in agarose gel was characterized by the appearance of an additional, fast-moving precipitin peak which was immunologically identical with the major, slower-moving VIIIR:Ag peak. The fast-moving peak was detected in all the patients with clinical conditions typically associated with increased plasma proteolysis (DIC, acute pancreatitis, during thrombolytic therapy). It was present in a smaller proportion of cases with liver and renal failure and malignancies and in the post-operative period. The additional VIIIR:Ag peak is thought to be the result of in vivo factor VIII/von Willebrand factor fragmentation by proteolytic enzymes.
在57例以急性期反应为特征的临床疾病患者中，VIII相关抗原（VIIIR:Ag）始终高于VIII凝血促酶活性（VIII:C）。两种不同的测量VIII:C的方法（一期和二期检测法）以及测量VIIIR:Ag的方法（电免疫扩散法和免疫放射分析）在大多数情况下结果一致。在43%的血浆样本中，琼脂糖凝胶交叉免疫电泳的特征是出现一个额外的、快速移动的沉淀素峰，其在免疫学上与主要的、移动较慢的VIIIR:Ag峰相同。在所有典型的与血浆蛋白水解增加相关的临床疾病患者（弥散性血管内凝血、急性胰腺炎、溶栓治疗期间）中均检测到快速移动峰。在肝功能衰竭、肾功能衰竭、恶性肿瘤患者以及术后患者中，出现该峰的比例较小。额外的VIIIR:Ag峰被认为是体内蛋白水解酶使VIII因子/血管性血友病因子片段化的结果。
