猪胰脂肪酶中与对硝基苯磷酸二乙酯反应的丝氨酸的特性研究。
Characterization of the serine reacting with diethyl p-nitrophenyl phosphate in porcine pancreatic lipase.
作者信息
Guidoni A, Benkouka F, De Caro J, Rovery M
出版信息
Biochim Biophys Acta. 1981 Jul 24;660(1):148-50. doi: 10.1016/0005-2744(81)90120-0.
PMID:6791692
Abstract
The position in porcine pancreatic lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) of the serine reacting specifically with emulsified or micellar diethyl p-nitrophenyl phosphate has been investigated. This serine which appears to be involved in lipase adsorption to insoluble triglyceride interfaces, is at position 152 in the enzyme chain. The sequence around this amino acid is: His-Val-Ile-Gly-His-Ser-Leu-Gly.
摘要
研究了猪胰脂肪酶（三酰甘油酰基水解酶，EC 3.1.1.3）中与乳化或胶束状对硝基苯基磷酸二乙酯特异性反应的丝氨酸的位置。这个似乎参与脂肪酶吸附到不溶性甘油三酯界面的丝氨酸，位于酶链的第152位。该氨基酸周围的序列是：组氨酸-缬氨酸-异亮氨酸-甘氨酸-组氨酸-丝氨酸-亮氨酸-甘氨酸。
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