人肝铁蛋白的三步纯化程序。
A three step purification procedure for human liver ferritin.
作者信息
Pagé M, Legueux J, Gauthier C
出版信息
Can J Biochem. 1980 Jun;58(6):494-8. doi: 10.1139/o80-066.
DOI:10.1139/o80-066
PMID:6791795
Abstract
We describe a method for the purification of normal human liver ferritin by ultrafiltration, gel filtration on Sephacryl S-300, and affinity chromatography on DEAE-Affi Gel Blue. The purity of the ferritin obtained was verified by immunoelectrophoresis, Ouchterlony immuno-diffusion, polyacrylamide gel electrophoresis, and electrofocusing. This rapid method yields 32% of the original ferritin.
摘要
我们描述了一种通过超滤、在Sephacryl S-300上进行凝胶过滤以及在DEAE-亲和蓝胶上进行亲和色谱来纯化正常人肝脏铁蛋白的方法。通过免疫电泳、双向免疫扩散、聚丙烯酰胺凝胶电泳和等电聚焦验证了所得铁蛋白的纯度。这种快速方法可得到原始铁蛋白的32%。
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