Lande S, Pawelek J, Lerner A B, Emanuel J R
J Invest Dermatol. 1981 Aug;77(2):244-5. doi: 10.1111/1523-1747.ep12480145.
A reproducible and sensitive assay for melanotropic agents is described employing mouse melanoma cells in culture and measuring tyrosinase activity in terms of production of tritiated water from L-(ring-3,5-3H)-tyrosine. Molar concentrations of peptides inducing one-half maximal stimulation of tyrosinase activity were: beta-MSH, 1 +/- 2 x 10(-9); alpha-MSH and Beta h-LPH, 1 +/- 2 x 10(-8); ACTHp, 1 +/- 2 x 10(-7). Beta p 9-18-MSH and melanotropin potentiating factor, beta s 88-91-LPH exhibited no activity at concentrations as high as 10(-5)M.
本文描述了一种可重复且灵敏的促黑素测定方法，该方法利用培养的小鼠黑色素瘤细胞，并根据从L-（环-3,5-³H）-酪氨酸产生的氚化水来测量酪氨酸酶活性。诱导酪氨酸酶活性达到最大刺激一半时的肽的摩尔浓度为：β-促黑素（β-MSH），1±2×10⁻⁹；α-促黑素（α-MSH）和β-人促脂素（β h-LPH），1±2×10⁻⁸；促肾上腺皮质激素原片段（ACTHp），1±2×10⁻⁷。β p 9-18-MSH和促黑素增强因子β s 88-91-LPH在高达10⁻⁵M的浓度下均无活性。
