Bulgakova V G, Petrykina Z M, Poltorak V A, Polin A N
Mikrobiologiia. 1981 May-Jun;50(3):498-503.
The carbonyl-conjugated pentaenes flavofungin, nigrofungin and flavopentin exhibit considerable lytic activity toward Micrococcus lysodeikticus and Bacillus megaterium protoplasts. The antibiotics at concentrations of 5 to 14 microgram/ml cause lysis of 50% of the protoplasts within 15 min of their incubation. The antibiotics inhibit the activity of NADH oxidase and malate oxidase by 50% in the lysates of Micrococcus lysodeikticus and Bacillus megaterium protoplasts at concentrations of 30 to 50 microgram/ml; preincubation of the lysates with the antibiotics intensify the inhibiting action of the polyenes. Growth of the bacteria is inhibited when the minimal concentration of the polyenes is 75 to 100 microgram/ml. Interaction of the polyenes with bacterial membranes lacking sterols indicates that resistance of at least some bacteria to polyenes is caused by impermeability of the cell wall for these substances rather than by the absence of sterols in the membranes.
羰基共轭戊二烯类抗生素黄曲霉素、黑曲霉素和黄曲戊菌素对溶壁微球菌和巨大芽孢杆菌原生质体具有显著的裂解活性。抗生素浓度为5至14微克/毫升时，在孵育15分钟内可使50%的原生质体裂解。抗生素浓度为30至50微克/毫升时，可使溶壁微球菌和巨大芽孢杆菌原生质体裂解物中的NADH氧化酶和苹果酸氧化酶活性抑制50%；裂解物与抗生素预孵育可增强多烯类的抑制作用。当多烯类最低浓度为75至100微克/毫升时，细菌生长受到抑制。多烯类与缺乏甾醇的细菌膜相互作用表明，至少某些细菌对多烯类的抗性是由于细胞壁对这些物质的不渗透性，而非膜中缺乏甾醇所致。
