Suarez-Kurtz G, Eastwood A B
Am J Physiol. 1981 Sep;241(3):C98-105. doi: 10.1152/ajpcell.1981.241.3.C98.
Isolated, intact frog muscles bathed in control saline release creatine kinase (CK) and lactate dehydrogenase (LDH) at constant rates for several hours. The basal rates of release from "toe" muscles (CK 0.087%/min; LDH 0.105%/min) were one order of magnitude greater than those from semitendinosus muscles. This is attributed to differences in muscle mass and geometry, and to the smaller diameter of toe muscle fibers. Enzyme release rates were not affected by Na-free or Cl-free solutions, whereas LDH release rate doubled during exposure to Ca-free (EGTA-containing) saline or in the presence of isosmotic solutions containing 120 mM KCl or potassium propionate. Following mechanical injury or detergent treatment (Brij 58), the enzyme release rates into Ca-free medium reached peak values 4 and 16 times (toe muscle), and 16 and 20-30 times (semitendinosus), respectively, the control rates. The greater effect of detergent treatment is ascribed to a larger area of sarcolemmal damage plus possible changes in the state of the enzymes in the sarcoplasm.
置于对照盐溶液中的分离完整蛙肌能在数小时内以恒定速率释放肌酸激酶（CK）和乳酸脱氢酶（LDH）。“趾”肌的基础释放速率（CK为0.087%/分钟；LDH为0.105%/分钟）比半腱肌的基础释放速率高一个数量级。这归因于肌肉质量和几何形状的差异，以及趾肌纤维直径较小。酶释放速率不受无钠或无氯溶液的影响，而在暴露于无钙（含乙二醇双四乙酸）盐溶液中或存在含120 mM氯化钾或丙酸钾的等渗溶液时，LDH释放速率加倍。在机械损伤或去污剂处理（Brij 58）后，酶向无钙培养基中的释放速率分别达到对照速率的4倍和16倍（趾肌），以及16倍和20 - 30倍（半腱肌）。去污剂处理的影响更大归因于肌膜损伤面积更大以及肌浆中酶状态可能发生的变化。
