Lawin-Brüssel C A, Refojo M F, Leong F L, Hanninen L, Kenyon K R
Eye Research Institute, Harvard Medical School, Boston, Mass.
Arch Ophthalmol. 1990 Jul;108(7):1012-9. doi: 10.1001/archopht.1990.01070090114052.
New perfilcon contact lenses (71% water) were placed on one eye of 36 rabbits under complete tarsorrhaphy for 7 days. The lenses were then removed, and 18 were incubated for 1 hour with a suspension of 1 x 10(8) colony-forming units per milliliter of Pseudomonas aeruginosa and replaced on their respective corneas. The remaining 18 eyes were inoculated with 0.1 mL of the P aeruginosa suspension; the lenses were not replaced. All eyes were again closed by tarsorrhaphy for 4, 8, 16, 24, 36 , or 48 hours. Ulcerative keratitis occurred in contact lens-wearing eyes only, as P aeruginosa penetrated the epithelium at 8 to 36 hours, and polymorphonuclear leukocyte migration began at 24 hours. Compared with conventional abrasion-wound models, the time course of infectious keratitis in this model was delayed, possibly due to less preexisting corneal trauma, but our findings suggest that soft contact lens wear facilitates bacterial invasion and also inhibits the host inflammatory response.
将含水量为71%的新型隐形眼镜置于36只兔子的一只眼睛上，在完全睑裂缝合的情况下放置7天。然后取出镜片，其中18只镜片与每毫升含1×10⁸ 菌落形成单位的铜绿假单胞菌悬液孵育1小时，再放回各自的角膜上。其余18只眼睛接种0.1 mL铜绿假单胞菌悬液；未更换镜片。所有眼睛再次通过睑裂缝合4、8、16、24、36或48小时。仅佩戴隐形眼镜的眼睛发生了溃疡性角膜炎，因为铜绿假单胞菌在8至36小时穿透上皮，多形核白细胞迁移在24小时开始。与传统的擦伤伤口模型相比，该模型中感染性角膜炎的时间进程有所延迟，可能是由于先前存在的角膜创伤较少，但我们的研究结果表明，佩戴软性隐形眼镜有利于细菌入侵，并且还会抑制宿主的炎症反应。
