Ziller C, Le Douarin N M, Brazeau P
C R Seances Acad Sci III. 1981 Jun 29;292(23):1215-9.
Mesencephalic neural crest cells from Quail embryos, cultivated in a defined serum free medium, exhibit neurite extension and typical neuronal traits. This differentiation is never achieved in the presence of serum. Addition of serum into the culture medium after neuronal differentiation has occurred, induces complete regression of the neurites.