Pinco O, Carmeli C, Rosenthal A, Kalcheim C
Department of Anatomy and Embryology, Hebrew University of Jerusalem-Hadassah Medical School, Israel.
J Neurobiol. 1993 Dec;24(12):1626-41. doi: 10.1002/neu.480241207.
Neurotrophin-3 is mitogenic for cultured quail neural crest cells (Kalcheim et al., 1992, Proc. Natl. Acad. Sci. USA 89:1661-1665). We now report that neurotrophin-3 also influences the survival and/or differentiation of a subset of postmitotic neural crest precursors into neurons, provided these progenitors are grown on a cellular substrate. When cultured for 1 day on monolayers of NT-3-producing, chinese hamster ovary cells, 59% of the neural crest clusters growing on the transfected line revealed the presence of intense neuronal outgrowth, compared to 25% of that in controls. Moreover, dissociated neural crest cells grown for 20 h on top of mesodermal cells in the presence of various concentrations of purified recombinant neurotrophin-3 displayed a dose-dependent increase in neuronal number. Localization experiments using specific polyclonal antibodies, revealed that neurotrophin-3 is confined to neuroepithelial cells of quail neural tubes in situ on E2 and E3, and to E2 neural tubes grown in culture for 24 h. At this stage, neural crest cells and somites were negative. At later stages, staining was likewise apparent in peripheral nerves and dorsal root ganglia. We, therefore, propose that NT-3, a factor that is expressed in the early avian central nervous system, has multiple effects both on the proliferation and differentiation of distinct neural crest cells, which depend on the state of commitment of the responsive progenitors.
神经营养因子-3对培养的鹌鹑神经嵴细胞具有促有丝分裂作用（卡尔海姆等人，1992年，《美国国家科学院院刊》89:1661 - 1665）。我们现在报告，神经营养因子-3也影响有丝分裂后神经嵴前体细胞亚群向神经元的存活和/或分化，前提是这些祖细胞生长在细胞基质上。当在产生NT - 3的中国仓鼠卵巢细胞单层上培养1天时，在转染细胞系上生长的神经嵴簇中有59%显示出强烈的神经元长出，而对照组中这一比例为25%。此外，在各种浓度的纯化重组神经营养因子-3存在下，解离的神经嵴细胞在中胚层细胞上生长20小时后，神经元数量呈剂量依赖性增加。使用特异性多克隆抗体的定位实验表明，神经营养因子-3在E2和E3期原位局限于鹌鹑神经管的神经上皮细胞，以及在培养24小时的E2神经管中。在此阶段，神经嵴细胞和体节呈阴性。在后期阶段，在外周神经和背根神经节中同样可见染色。因此，我们提出，在早期鸟类中枢神经系统中表达的NT - 3因子，对不同神经嵴细胞的增殖和分化具有多种作用，这取决于反应性祖细胞的分化状态。
