Huck S, Hatten M E
J Neurosci. 1981 Oct;1(10):1075-84. doi: 10.1523/JNEUROSCI.01-10-01075.1981.
Eleven fluorescein isothiocyanate-conjugated (FITC) lectins, each with distinct carbohydrate-binding properties, were used to assess cell surface glycoconjugates of embryonic and early postnatal cerebellar cells in vitro. Fluorescence staining of embryonic day 13 (E13) cerebellar cells with FITC Ricinus communis agglutinin diminished markedly between 24 and 72 hr in vitro. No staining of postnatal day 0 (P0) or postnatal day 7 (P7) cells was observed with FITC Ricinus communis agglutinin. A similar, but less pronounced decrease in FITC concanavalin A, FITC Lens culinaris, and FITC wheat germ agglutinin was observed between embryonic day 13 and birth. No specific staining of E13, P0, or P7 cultures was observed with FITC peanut agglutinin, FITC Dolichos bifloris agglutinin, FITC soybean agglutinin, FITC Wistaria floribundis agglutinin, FITC Phaseolus vulgaris agglutinin, FITC Limulus polyphemus agglutinin, or FITC Ulex europaeusI agglutinin. Similar results were obtained with 125I-lectin binding assays. Ricinus communis 125-I-agglutinin binding decreased dramatically between embryonic day 13 and birth. Less pronounced decreases were observed in 125I-concanavalin A and wheat germ 125I-agglutinin binding. Very low levels of soybean 125I-agglutinin or Ulex europaeusI 125I-agglutinin were bound by either embryonic or early postnatal cerebellar cells in vitro.
使用了11种异硫氰酸荧光素偶联（FITC）凝集素，每种凝集素都具有独特的碳水化合物结合特性，用于体外评估胚胎期和出生后早期小脑细胞的细胞表面糖缀合物。体外培养24至72小时期间，用FITC蓖麻凝集素对胚胎第13天（E13）的小脑细胞进行荧光染色明显减弱。未观察到出生后第0天（P0）或出生后第7天（P7）的细胞被FITC蓖麻凝集素染色。在胚胎第13天至出生之间，观察到FITC伴刀豆球蛋白A、FITC小扁豆凝集素和FITC麦胚凝集素也有类似但不太明显的减少。未观察到FITC花生凝集素、FITC双花扁豆凝集素、FITC大豆凝集素、FITC多花紫藤凝集素、FITC菜豆凝集素、FITC鲎凝集素或FITC荆豆I凝集素对E13、P0或P7培养物有特异性染色。125I-凝集素结合试验也得到了类似的结果。蓖麻125-I-凝集素结合在胚胎第13天至出生之间急剧下降。观察到125I-伴刀豆球蛋白A和麦胚125I-凝集素结合的下降不太明显。体外培养时，胚胎期或出生后早期的小脑细胞与大豆125I-凝集素或荆豆I 125I-凝集素的结合水平都非常低。
