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用于分析免疫化学技术的多层凝胶板方法。

The method of multilayer gel plates for the analytical immunochemical techniques.

作者信息

Constantinescu G, Simionescu L, Teodorescu-Exarcu I

出版信息

Endocrinologie. 1981 Jul-Sep;19(3):195-8.

PMID:6794142
Abstract

A new analytical immunochemical method using gel film copies obtained by means of multilayer gel plates is described. The method is suitable for any variant of immunochemical gel technique. Perfect identity of the copies as well as some advantages are exemplified. In comparison with the already known monolayer gel plate method, the multilayer plate is more advantageous for the study of the dynamics of the antigen-antibody reaction, for the discovery of the nature of some unknown biological products available in very low quantities and for the discovery of the nature of some unknown biological products available in very low quantities and for the economy of certain costly reagents. Moreover, its high reproductibility recommends it as a reference method for the control of quality, especially of the precision and accuracy of the monolayer immunofiltration techniques.

摘要

本文描述了一种新的分析免疫化学方法,该方法使用通过多层凝胶板获得的凝胶膜复制品。该方法适用于免疫化学凝胶技术的任何变体。文中举例说明了复制品的完美一致性以及一些优点。与已知的单层凝胶板方法相比,多层板在研究抗原 - 抗体反应动力学、发现极少量存在的某些未知生物产物的性质以及节省某些昂贵试剂方面更具优势。此外,其高重现性使其成为质量控制的参考方法,尤其是用于控制单层免疫过滤技术的精密度和准确性。

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