Boeynaems J M, Pelster D, Oates J A, Hubbard W C
Biochim Biophys Acta. 1981 Sep 24;665(3):623-7. doi: 10.1016/0005-2760(81)90281-2.
The transformation of arachidonic acid by the rat thyroid in vitro has been investigated. At least two metabolites have been partially characterized: they differed from known metabolites of arachidonic acid in terms of retention volume in liquid chromatography, ultraviolet spectrophotometry and pharmacology (formation not inhibited by indomethacin and enhanced by eicosatetraynoic acid). The analysis by chemical ionization mass spectrometry suggested that these metabolites might be diketo-monohydroxy- and monoketo-dihydroxy-compounds. The conversion of arachidonic acid into these compounds was stimulated by ionophore A23187, decreased by the peroxidase inhibitor methimazole and potentiated by iodide, suggesting that this pathway is under the control of Ca2+ and of a peroxidase product.
已对大鼠甲状腺在体外对花生四烯酸的转化进行了研究。至少已对两种代谢产物进行了部分表征：就液相色谱中的保留体积、紫外分光光度法和药理学而言（其形成不受吲哚美辛抑制且被二十碳四炔酸增强），它们与花生四烯酸的已知代谢产物不同。化学电离质谱分析表明，这些代谢产物可能是二酮单羟基和单酮二羟基化合物。离子载体A23187可刺激花生四烯酸转化为这些化合物，过氧化物酶抑制剂甲巯咪唑可使其减少，碘化物可使其增强，这表明该途径受Ca2+和一种过氧化物酶产物的控制。
