Rehak N N, Young D S
Clin Chem. 1978 Aug;24(8):1414-9.
Calorimetric analysis depends on the direct proportionality between the heat changes that occur during chemical reactions and the amount of reacting substances. Potential uses of calorimetry in the clinical laboratory are discussed, with examples. The calorimetric technique does not require optically clear specimens, and if the specificity of the measured reaction is assured, calorimetry can be used for quantitative determinations of components that are present in a complex matrix system such as body fluids. Specific enzymic reactions have been used to measure substrates and enzyme activities in biological specimens calorimetrically, with precision, sensitivity, and accuracy comparable to routine photometric techniques. The application of calorimetry in the clinical laboratory is limited now by its slowness, but development of automated instruments may enable the technique to become competitive with conventional analytical techniques in the clinical laboratory.
量热分析取决于化学反应过程中发生的热变化与反应物量之间的正比关系。文中讨论了量热法在临床实验室的潜在用途，并给出了示例。量热技术不需要光学透明的样本，并且如果所测反应的特异性有保证，量热法可用于定量测定存在于复杂基质系统（如体液）中的成分。特定的酶促反应已被用于通过量热法精确、灵敏且准确地测量生物样本中的底物和酶活性，其与常规光度技术相当。目前，量热法在临床实验室的应用因速度慢而受到限制，但自动化仪器的发展可能使该技术在临床实验室中能够与传统分析技术竞争。
