An improved method for the determination of human erythrocyte pyrimidine 5'-nucleotidase activity.
作者信息
Ellims P H, Bailey L, Van der Weyden M B
出版信息
Clin Chim Acta. 1978 Aug 15;88(1):99-103. doi: 10.1016/0009-8981(78)90155-9.
PMID:679498
Abstract
A radiometric method is described for measuring pyrimidine 5'-nucleotidase activity in human peripheral blood haemolysate. The substrate [14C]uridine monophosphate is converted to [14C]uridine. Two simple and rapid methods to isolate the product of the reaction employing either descending chromatography or elution from DEAE cellulose paper have been developed. With the described methods the specific activity of pyrimidine 5'-nucleotidase of human erythrocytes is 6.7 +/- 2.7 (+/- 1 S.D.) nmol/h/mg protein and this activity appears to be widely distributed in human tissue.
摘要
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