免疫球蛋白轻链信使核糖核酸由大核核糖核酸加工而成。
Immunoglobulin light chain mRNA is processed from large nuclear RNA.
作者信息
Gilmore-Hebert M, Wall R
出版信息
Proc Natl Acad Sci U S A. 1978 Jan;75(1):342-5. doi: 10.1073/pnas.75.1.342.
Abstract
Recombinant DNA probes, produced by the molecular cloning of immunoglobulin kappa light chain mRNA, have been used to analyze heterogenous nuclear RNA for presumptive precursors to cytoplasmic kappa light chain mRNA, Three discrete classes of nuclear RNA containing kappa mRNA sequences were detected after pulse-labeling of immunoglobulin-producing P3 myeloma cells. Two of these were substantially larger than kappa mRNA (approximately 10 and 4 times larger); the third was similar in size to kappa mRNA. Beginning with the largest, the sequential appearance of these three classes of nuclear RNA preceded the first appearance of newly synthesized kappa light chain mRNA in the cytoplasm. The results presented here suggest that immunoglobulin kappa light chain mRNA is generated by the stepwise cleavage and processing of a large nuclear RNA transcript.
摘要
通过免疫球蛋白κ轻链mRNA的分子克隆产生的重组DNA探针，已被用于分析不均一核RNA，以寻找细胞质κ轻链mRNA的推定前体。在用产生免疫球蛋白的P3骨髓瘤细胞进行脉冲标记后，检测到三类含有κmRNA序列的离散核RNA。其中两类比κmRNA大得多（大约大10倍和4倍）；第三类大小与κmRNA相似。从最大的开始，这三类核RNA的相继出现先于细胞质中新合成的κ轻链mRNA的首次出现。此处给出的结果表明，免疫球蛋白κ轻链mRNA是由一个大的核RNA转录本逐步切割和加工产生的。
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