Nakayasu M, Fujiki H, Mori M, Sugimura T, Moore R E
Cancer Lett. 1981 May;12(4):271-7. doi: 10.1016/0304-3835(81)90168-3.
Teleocidin isolated from Streptomyces mediocidicus, its catalytically hydrogenated compound dihydroteleocidin B, and lyngbyatoxin A isolated from marine blue-green alga Lyngbya majuscula as well as its hydrogenated product, tetrahydrolyngbyatoxin A were tested for their ability to induce differentiation of human promyelocytic leukemia cells (HL-60 cells) in culture. All of these indole alkaloids induced differentiation of HL-60 cells, characterized by increased phagocytosis, increased release of lysozyme, and morphological changes resembling macrophages. The concentrations required for the induction were 1-5 ng/ml, showing similarity to those of 12-O-tetradecanoylphorbol-13-acetate (TPA). Teleocidin, lyngbyatoxin A, and tetrahydrolyngbyatoxin A are suggested to be tumor promoters, like TPA and dihydroteleocidin B. The HL-60 cell system might be useful for screening for environmental tumor promoters.
对从平庸链霉菌中分离得到的远侧素、其催化氢化化合物二氢远侧素B、从海洋蓝绿藻巨大鞘丝藻中分离得到的林加毒素A及其氢化产物四氢林加毒素A在培养条件下诱导人早幼粒细胞白血病细胞（HL-60细胞）分化的能力进行了测试。所有这些吲哚生物碱均能诱导HL-60细胞分化，其特征为吞噬作用增强、溶菌酶释放增加以及出现类似巨噬细胞的形态变化。诱导所需的浓度为1 - 5纳克/毫升，与12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）的浓度相似。远侧素、林加毒素A和四氢林加毒素A被认为与TPA和二氢远侧素B一样是肿瘤促进剂。HL - 60细胞系统可能有助于筛选环境肿瘤促进剂。
