Huang R J, Liau M Y, Lee J D
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1981 Jun;14(2):92-5.
The neutralizing antibody titre of tetanus antitoxin could be elevated 3.19 times by the purification of the antitoxin with pepsin digestion method, which is superior to ammonium sulfate salting out method by 1.5 times. But the antitoxin recovery rate by the pepsin digestion method was about 65.37% of the salting out method. Addition of 2% glycine significantly improved the solubility of lyophilized antitoxin. The effective period of liquid antitoxin was 1 year, while that of lyophilized products could be as long as 5 years.
用胃蛋白酶消化法纯化破伤风抗毒素，其中和抗体效价可提高3.19倍，比硫酸铵盐析法高1.5倍。但胃蛋白酶消化法的抗毒素回收率约为盐析法的65.37%。添加2%甘氨酸可显著提高冻干抗毒素的溶解度。液体抗毒素的有效期为1年，而冻干制品的有效期可达5年。
