Cole L A, Hussa R O
Endocrinology. 1981 Dec;109(6):2276-8. doi: 10.1210/endo-109-6-2276.
Recent studies have demonstrated that ectopic glycoprotein hormones only partially bind Con A. To investigate the basis for these findings, the Con A binding of ectopic hCG beta from DoT cervical carcinoma cells was examined after digestion with various glycosidases. Ectopic hCG beta only partially bound Con A, as was the case after digestion with neuraminidase and beta-galactosidase. However, subsequent digestion with N-acetylhexosaminidase increased Con A binding to 96%. It is apparent that Con A binding of ectopic hCG can be inhibited by a residue removed by N-acetylhexosaminidase, probably extra beta-N-acetylglucosamine linked to beta-mannose on N-linked oligosaccharides. The method used involved the glycosidase digestion of glycoprotein alkylated under denaturing conditions and was first validated with milligram amounts of standard hCG beta.
最近的研究表明，异位糖蛋白激素仅部分结合伴刀豆球蛋白A（Con A）。为了探究这些发现的依据，在用各种糖苷酶消化后，检测了来自DoT宫颈癌细胞的异位人绒毛膜促性腺激素β亚基（hCGβ）与Con A的结合情况。异位hCGβ仅部分结合Con A，用神经氨酸酶和β-半乳糖苷酶消化后也是如此。然而，随后用N-乙酰己糖胺酶消化使Con A结合增加到96%。很明显，N-乙酰己糖胺酶去除的一个残基可抑制异位hCG与Con A的结合，该残基可能是与N-连接寡糖上的β-甘露糖相连的额外β-N-乙酰葡糖胺。所使用的方法涉及在变性条件下对糖蛋白进行烷基化处理后用糖苷酶消化，并且首先用毫克量的标准hCGβ进行了验证。
