MacGregor J T, Wehr C M, Gould D H
Environ Mutagen. 1980;2(4):509-14. doi: 10.1002/em.2860020408.
Micronuclei induced in bone marrow erythroblasts by clastogenic chemicals are easily detected in peripheral blood. In mice treated with nitrogen mustard, 7,12 dimethylbenz(a)anthracene, or cyclophosphamide, the peak incidence of micronucleated polychromatic erythrocytes in peripheral blood was at least as great as the maximum incidence in bone marrow. In each case the peak incidence in blood occurred on the day following the peak incidence observed in bone marrow. Thus, for general genetic screening purposes, monitoring micronuclei in peripheral blood rather than in bone marrow smears provides at least equal sensitivity, offers greater simplicity in sample preparation and scoring, permits multiple sampling of treated animals, and may also facilitate automated scoring and human cytogenetic monitoring.
致断裂化学物质在骨髓成红细胞中诱导产生的微核很容易在外周血中检测到。在用氮芥、7,12 - 二甲基苯并(a)蒽或环磷酰胺处理的小鼠中，外周血中微核多染性红细胞的峰值发生率至少与骨髓中的最大发生率一样高。在每种情况下，血液中的峰值发生率都出现在骨髓中观察到的峰值发生率之后的一天。因此，出于一般遗传筛查目的，监测外周血中的微核而非骨髓涂片至少具有同等的敏感性，在样品制备和评分方面更简单，允许对处理过的动物进行多次采样，并且还可能便于自动评分和人类细胞遗传学监测。
