Goldberg M T, Blakey D H, Bruce W R
Mutat Res. 1983 Apr;109(1):91-8. doi: 10.1016/0027-5107(83)90098-2.
An in vivo micronucleus assay has been developed that utilizes colonic epithelial cells. The genotoxic effects of 1,2-dimethylhydrazine (54-07-3), a colon carcinogen, and of the nitrogen mustard, cyclophosphamide (50-18-0), on the bone-marrow polychromatic erythrocytes and on colonic epithelium from mice were compared using micronucleus induction in each organ as the end point. In the bone marrow, cyclophosphamide was a potent inducer of micronuclei, while 1,2-dimethylhydrazine administration had little effect on the micronucleus incidence. In the colon, 1,2-diemthylhydrazine was an effective inducer of micronuclei. Thus, the colonic micronucleus assay appears to be a potentially useful test for the detection of colon carcinogens.
已开发出一种利用结肠上皮细胞的体内微核试验。以每个器官中的微核诱导为终点，比较了结肠致癌物1,2 - 二甲基肼（54 - 07 - 3）和氮芥环磷酰胺（50 - 18 - 0）对小鼠骨髓嗜多染红细胞和结肠上皮的遗传毒性作用。在骨髓中，环磷酰胺是微核的强效诱导剂，而给予1,2 - 二甲基肼对微核发生率影响很小。在结肠中，1,2 - 二甲基肼是微核的有效诱导剂。因此，结肠微核试验似乎是检测结肠致癌物的一种潜在有用的试验。
