Bryant D A, Cohen-Bazire G
Eur J Biochem. 1981 Oct;119(2):415-24. doi: 10.1111/j.1432-1033.1981.tb05624.x.
Pseudanabaena 7409 is a chromatically cyanobacterium which photocontrols the synthesis of both phycoerythrin and phycocyanin [Tandeau de Marsac (1977) J. Bacteriol. 130, 82--91]. Phycobilisomes, isolated from cells grown in either green or red light, have been dissociated and the component biliproteins purified and characterized. Phycobilisomes isolated from cells grown in green light were composed of allophycocyanin B, allophycocyanin, two phycocyanin subunits (one alpha-type and one beta-type subunit), phycoerythrin and eight uncolored polypeptides. When dissociated phycobilisomes were chromatographed on DEAE-cellulose at pH 5.5, most of the phycocyanin was recovered as part of a large (17.3 S) multiprotein complex with phycoerythrin (molar ratio 1 : 1). This complex also contained five of the uncolored polypeptides found in intact phycobilisomes isolated from cells grown in green light. Phycobilisomes isolated from cells grown in red light were composed of allophycocyanin B, allophycocyanin, four phycocyanin subunits (two alpha-type and two beta-type subunits), and six uncolored polypeptides. When these phycobilisomes were dissociated, the phycocyanin was recovered as a large (21.0 S) multiprotein complex which was composed of the four phycocyanin subunits types and four uncolored polypeptides. This complex was morphologically identical to the rod-like stacks of discs about 6 x 12 nm which form the peripheral rods of intact phycobilisomes. Each of the four phycocyanin subunits found in the complex isolated from the phycobilisomes of cells grown in red light was purified to homogeneity and characterized. Amino acid compositions of the four subunits indicated that each subunit was a unique gene product. Two of the subunits of the complex were apparently identical to those of the phycocyanin purified from phycobilisomes isolated from cells grown in green light. These studies suggest that one pair of phycocyanin subunits was synthesized constitutively (i.e. irrespective of the light wavelength to which the cells were exposed during growth) while the synthesis of the second pair of phycocyanin subunits was specifically induced during growth in red light.
假鱼腥藻7409是一种能进行色素变化的蓝细菌，它能光控藻红蛋白和藻蓝蛋白的合成[Tandeau de Marsac（1977年）《细菌学杂志》130卷，82 - 91页]。从在绿光或红光下生长的细胞中分离出的藻胆体已被解离，其组成的胆色素蛋白已被纯化并进行了特性分析。从在绿光下生长的细胞中分离出的藻胆体由别藻蓝蛋白B、别藻蓝蛋白、两个藻蓝蛋白亚基（一个α型亚基和一个β型亚基）、藻红蛋白和8种无色多肽组成。当将解离后的藻胆体在pH 5.5的DEAE - 纤维素上进行层析时，大部分藻蓝蛋白作为一个大的（17.3 S）多蛋白复合物的一部分被回收，该复合物与藻红蛋白（摩尔比1 : 1）结合。这个复合物还包含从在绿光下生长的细胞中分离出的完整藻胆体中发现的5种无色多肽。从在红光下生长的细胞中分离出的藻胆体由别藻蓝蛋白B、别藻蓝蛋白、4个藻蓝蛋白亚基（两个α型亚基和两个β型亚基）和6种无色多肽组成。当这些藻胆体被解离时，藻蓝蛋白作为一个大的（21.0 S）多蛋白复合物被回收，该复合物由4种藻蓝蛋白亚基类型和4种无色多肽组成。这个复合物在形态上与完整藻胆体的外周棒状结构中形成的约6×12 nm的盘状杆状堆叠相同。从在红光下生长的细胞的藻胆体中分离出的复合物中发现的4种藻蓝蛋白亚基中的每一种都被纯化至同质并进行了特性分析。这4个亚基的氨基酸组成表明每个亚基都是一个独特的基因产物。该复合物中的两个亚基显然与从在绿光下生长的细胞中分离出的藻胆体中纯化的藻蓝蛋白的亚基相同。这些研究表明，一对藻蓝蛋白亚基是组成性合成的（即无论细胞在生长过程中暴露于何种波长的光下），而另一对藻蓝蛋白亚基的合成是在红光下生长期间特异性诱导的。
