前列腺素F2α的β-半乳糖苷酶联免疫测定

beta-Galactosidase-linked immunoassay of prostaglandin F2 alpha.

作者信息

Yano T, Hayashi Y, Yamamoto S

出版信息

J Biochem. 1981 Sep;90(3):773-7. doi: 10.1093/oxfordjournals.jbchem.a133532.

DOI:10.1093/oxfordjournals.jbchem.a133532

Abstract

A sensitive and specific enzyme immunoassay for prostaglandin F2 alpha was developed in which prostaglandin F2 alpha was labeled with beta-D-galactosidase. After competitive binding to antibody between enzyme-labeled and free prostaglandin F2 alpha, the immunoreactive product was precipitated by double antibody technique, and the enzyme activity of the precipitate was determined fluorometrically. The procedures allowed determination of 0.03-20 pmol of prostaglandin F2 alpha. The detection limit of 0.03 pmol was comparable with that of previously reported radioimmunoassays. Enzyme immunoassay and radioimmunoassay showed almost the same cross-reactivity with other prostaglandins and metabolites.

摘要

开发了一种用于前列腺素F2α的灵敏且特异的酶免疫测定法，其中前列腺素F2α用β-D-半乳糖苷酶标记。在酶标记的和游离的前列腺素F2α与抗体竞争性结合后，采用双抗体技术使免疫反应产物沉淀，并用荧光法测定沉淀物的酶活性。该方法可测定0.03 - 20 pmol的前列腺素F2α。0.03 pmol的检测限与先前报道的放射免疫测定法相当。酶免疫测定法和放射免疫测定法对其他前列腺素和代谢产物显示出几乎相同的交叉反应性。