一种以过氧化物酶为标记物的血栓素B2和6-氧代前列环素F1α的酶联免疫分析方法。
An enzyme-linked immunoassay for thromboxane B2 and 6-oxo-PGF1 alpha using peroxidase as label.
作者信息
Giessler C, Panse M, Mentz P, Hellthaler G
机构信息
Department of Pharmacology and Toxicology, Martin Luther University Halle-Wittenberg, GDR.
出版信息
Biomed Biochim Acta. 1988;47(10-11):S137-9.
A sensitive and specific enzyme immunoassay was developed for TXB2 and 6-oxo-PGF1 alpha, stable degradation products of TXA2 and prostacyclin. The hapten molecule was labeled with pure peroxidase. After competitive binding to antibody between enzyme-labeled and free prostanoids the immunoreactive complex was precipitated by double antibody technique. The enzyme activity of the precipitate was determined spectrophotometrically. The procedure allowed a determination of TXB2 and 6-oxo-PGF1 alpha in the range of 3-200 pg (0.008-0.53 pmol) and 10-1000 pg (0.028-2.8 pmol) respectively.
针对血栓素A2（TXA2）和前列环素的稳定降解产物TXB2和6-氧代前列腺素F1α，开发了一种灵敏且特异的酶免疫测定法。半抗原分子用纯过氧化物酶标记。酶标记的前列腺素和游离前列腺素与抗体竞争性结合后，通过双抗体技术沉淀免疫反应复合物。用分光光度法测定沉淀物的酶活性。该方法分别可测定3 - 200皮克（0.008 - 0.53皮摩尔）范围内的TXB2和10 - 1000皮克（0.028 - 2.8皮摩尔）范围内的6-氧代前列腺素F1α。
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