Păuşescu E
Physiologie. 1981;18(3):213-25 contd.
Since there are controversial data and opinions on the use of lactate as a viability test for preserved hearts, investigations were carried out of the variations of lactate and pyruvate amounts in the perfusion fluid during hypothermic preservation of canine hearts by means of a perfusion fluid containing amino acids in "cytoplasmic" concentrations. These variations were interpreted in connection with the changes in the coronary perfusion flow during storage and the functional performances of the preserved hearts during long periods of subsequent ex vivo functional testing. Likewise, investigations of the ability of the myocardial mitochondria to oxidize in vitro lactate and pyruvate at low temperature levels were performed in order to explain the nature of the impairment in using these metabolic substrates during preservation and of the functional cardiac disturbances after resuscitation. The investigations have led to the conclusion that lactate should not be used as the sole index of heart storage efficiency and that it gains in significance only when a high myocardial lactate production is associated with a progressively increasing resistance in the coronary vasculature during preservation. The possible metabolic significance of the results obtained in these investigations are amply discussed.
由于在将乳酸用作保存心脏的活力测试方面存在有争议的数据和观点，因此开展了相关研究，通过使用含有“细胞质”浓度氨基酸的灌注液，对犬心低温保存期间灌注液中乳酸和丙酮酸含量的变化进行研究。这些变化结合保存期间冠状动脉灌注流量的变化以及随后长时间体外功能测试中保存心脏的功能表现进行解读。同样，为了解释保存期间使用这些代谢底物时的损伤性质以及复苏后心脏功能紊乱的情况，还对心肌线粒体在低温水平下体外氧化乳酸和丙酮酸的能力进行了研究。这些研究得出的结论是，乳酸不应被用作心脏保存效率的唯一指标，只有当心肌乳酸生成量高且保存期间冠状动脉血管阻力逐渐增加时，乳酸才具有重要意义。文中充分讨论了这些研究所获结果可能具有的代谢意义。
