Yamane N, Odagiri T, Arikawa J, Ishida N
Tohoku J Exp Med. 1981 Mar;133(3):245-55. doi: 10.1620/tjem.133.245.
The reversed single-radial-immunodiffusion (r-SRD) test using the avian-origin influenza A virus, A/chicken/Germany "N'/49 (Hav2Neql) disrupted with 1.0% sarkosyl, was established as the quantitative method for the assay of a type-specific antibody, particularly of an anti-nucleoprotein antibody, in human sera. Under the test conditions, human sera produced a definite opalescent zone around the well and the annulus area was found to have a high correlation to the antibody level of type A influenza nucleoprotein. The specificity of the opalescent zone produced in the test plate was confirmed with the specific antisera to each viral polypeptide and the adsorption procedures with the purified nucleoprotein. During an epidemic of H3N2 and H1N1 viruses, the r-SRD test was employed to estimate the infection ratio in junior high school students. The results demonstrated its convenience and high sensitivity in detecting the antibody rise to influenza A nucleoprotein. In addition, the age-distribution of the antibody level to influenza A nucleoprotein was examined and discussed. The results obtained strongly suggested that the r-SRD technique using the avian-origin influenza A virus provided a simple and reproducible method for the assay of the antibody level to influenza A nucleoprotein in a large scale seroepidemiology and in the serodiagnosis of influenza A virus infection.
使用经1.0% Sarkosyl裂解的禽源甲型流感病毒A/鸡/德国“N'/49（Hav2Neql）建立的反向单放射免疫扩散（r - SRD）试验，被确立为检测人血清中特异性抗体，特别是抗核蛋白抗体的定量方法。在试验条件下，人血清在孔周围产生明确的乳白色区域，发现环带面积与甲型流感核蛋白抗体水平高度相关。用针对每种病毒多肽的特异性抗血清以及用纯化核蛋白进行吸附程序，证实了试验板中产生的乳白色区域的特异性。在H3N2和H1N1病毒流行期间，采用r - SRD试验来估计初中生的感染率。结果证明其在检测甲型流感核蛋白抗体升高方面具有便利性和高灵敏度。此外，还对甲型流感核蛋白抗体水平的年龄分布进行了检查和讨论。所获得的结果有力地表明，使用禽源甲型流感病毒的r - SRD技术为大规模血清流行病学和甲型流感病毒感染的血清诊断中甲型流感核蛋白抗体水平的检测提供了一种简单且可重复的方法。
