Korpela H, Hölttä E, Hovi T, Jänne J
Biochem J. 1981 Jun 15;196(3):733-8. doi: 10.1042/bj1960733.
The stimulation of lymphocyte ornithine decarboxylase and adenosylmethionine decarboxylase produced by phytohaemagglutinin was accompanied by an equally marked, but delayed, stimulation of spermidine synthase, which is not commonly considered as an inducible enzyme. In contrast with the marked stimulation of these biosynthetic enzymes, less marked changes were observed in the biodegradative enzymes of polyamines in response to phytohaemagglutinin. Diamine oxidase activity was undetectable during all stages of the transformation. The activity of polyamine oxidase remained either constant or was slightly decreased several days after addition of the mitogen. The activity of polyamine acetylase (employing all the natural polyamines as substrates) distinctly increased both in the cytosolic and crude nuclear preparations of the cells during later stages of mitogen activation. Difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, although powerfully inhibiting ornithine decarboxylase, produced a gradual enhancement of adenosylmethionine decarboxylase activity during lymphocyte activation, without influencing the activities of the two propylamine transferases (spermidine synthase and spermine synthase).
植物血凝素对淋巴细胞鸟氨酸脱羧酶和腺苷甲硫氨酸脱羧酶的刺激，伴随着亚精胺合酶同样显著但延迟的刺激，亚精胺合酶通常不被认为是一种可诱导酶。与这些生物合成酶的显著刺激相反，观察到多胺的生物降解酶对植物血凝素的反应变化较小。在转化的所有阶段均未检测到二胺氧化酶活性。添加促有丝分裂原几天后，多胺氧化酶的活性保持恒定或略有下降。在促有丝分裂原激活的后期，多胺乙酰酶（以所有天然多胺为底物）的活性在细胞的胞质和粗核制剂中均明显增加。二氟甲基鸟氨酸是鸟氨酸脱羧酶的不可逆抑制剂，尽管它能强烈抑制鸟氨酸脱羧酶，但在淋巴细胞激活过程中会使腺苷甲硫氨酸脱羧酶的活性逐渐增强，而不影响两种丙胺转移酶（亚精胺合酶和精胺合酶）的活性。
