Musquera S, Delers F, Lombart C
C R Seances Acad Sci III. 1981 Oct 19;293(6):313-8.
A one step procedure for the purification of a Chicken hemoglobin binding protein is reported here for the first time. The method includes a single affinity chromatography step on Sepharose coupled to Chicken hemoglobin. The protein obtained is pure, its ability of binding hemoglobin is maintained and the recovery is close to 100%. Moreover, the same affinity system may be used again.
本文首次报道了一种纯化鸡血红蛋白结合蛋白的一步法。该方法包括在偶联有鸡血红蛋白的琼脂糖上进行单步亲和层析。所获得的蛋白质是纯的，其结合血红蛋白的能力得以保持，回收率接近100%。此外，相同的亲和系统可以再次使用。
