Lees S, Heeley J D
Calcif Tissue Int. 1981;33(5):499-504. doi: 10.1007/BF02409480.
The density of a bovine cortical bone matrix sample was found in water, several ethanol-water solutions, and in dried state. Previously the density of the same mineralized bone was found fresh and when desiccated. The volume in each state was estimated from the dimensional changes axially, tangentially, and radially. Confirmation was found by determining the density of dried specimens upon immersion in xylene. The amount of imbibed xylene provided an estimate of the free pore volume in the dried matrix. The volume fraction of the solid constituent, S, in the wet matrix was found to be 0.57, from which the density of S in various solutions was calculated. Density of wet matrix in 0.15 M saline: 1.180 g/cc; for dried matrix, 1.246 g/cc. Density of wet S in saline: 1.33 g/cc; for dried S, 1.42 g/cc, which matches published values for collagen molecules. Dimensional changes between wet and dried state of matrix match published values for artificially cross-linked rat tail tendon fibers. Axially: 1.04, by area: 2.27; by volume: 2.62. Estimate of intrafibrillar volume, assuming 80% of mineral is within fibrils: 0.73 cc/g dry collagen.
在水中、几种乙醇 - 水溶液以及干燥状态下测定了牛皮质骨基质样本的密度。此前已测定了相同矿化骨新鲜时以及干燥后的密度。通过轴向、切向和径向的尺寸变化估算每种状态下的体积。将干燥标本浸入二甲苯后测定其密度以进行验证。吸收的二甲苯量可估算干燥基质中的自由孔隙体积。发现湿基质中固体成分S的体积分数为0.57，并据此计算了S在各种溶液中的密度。在0.15 M盐水中湿基质的密度：1.180 g/cc；干燥基质的密度为1.246 g/cc。盐水中湿S的密度：1.33 g/cc；干燥S的密度为1.42 g/cc，这与已发表的胶原蛋白分子的值相符。基质湿态和干态之间的尺寸变化与人工交联的大鼠尾腱纤维的已发表值相符。轴向：1.04，面积方向：2.27；体积方向：2.62。假设80%的矿物质在纤维内，纤维内体积的估算值：0.73 cc/g干胶原蛋白。
