Inoue Y, Nagasawa K
Carbohydr Res. 1981 Nov 16;97(2):263-78. doi: 10.1016/s0008-6215(00)80672-2.
Nonsulfated di- to octadeca-saccharides having 2-acetamido-2-deoxy-D-galactose at the reducing end were prepared, in 81% yield, by treatment of chondroitin 6-sulfate (pyridinium salt) with dimethyl sulfoxide containing 10% of water for 14 h at 90 degrees. N-Acetylchondrosine and N-acetyldermosine were obtained from dermatan sulfate of rooster comb, in 30% and 38% yields, respectively, by solvolysis with dimethyl sulfoxide, containing 10% of water, for 30 h at 105 degrees. Hyaluronic acid was also depolymerized by the same solvent in the presence of an equimolar amount of pyridinium sulfate or chloride per disaccharide unit to give reducing di- and higher molecular weight oligo-saccharides. The results of solvolytic desulfation and depolymerization are compared with those of the conventional methods by acid hydrolysis.
通过在90℃下用含10%水的二甲基亚砜处理硫酸软骨素6 - 硫酸酯（吡啶盐）14小时，以81%的产率制备了还原端具有2 - 乙酰氨基 - 2 - 脱氧 - D - 半乳糖的非硫酸化二糖至十八糖。通过在105℃下用含10%水的二甲基亚砜进行溶剂解30小时，分别从公鸡鸡冠的硫酸皮肤素中获得N - 乙酰软骨素和N - 乙酰皮肤素，产率分别为30%和38%。在每二糖单元存在等摩尔量的硫酸吡啶盐或氯化物的情况下，透明质酸也被相同的溶剂解聚，得到还原性二糖和更高分子量的寡糖。将溶剂解脱硫和解聚的结果与通过酸水解的传统方法的结果进行了比较。
