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枯草芽孢杆菌质粒转化和转导中的“标记增强”现象

["Marker augmentation" phenomenon in the plasmid transformation and transduction of Bacillus subtilis].

作者信息

Bashkirov V I, Lakomova N M, Surikov N N, Prozorov A A

出版信息

Genetika. 1981;17(11):1929-35.

PMID:6797871
Abstract

Transformation of Bacillus subtilis cells carrying pUB110 plasmid by DNA of homologous plasmid pBD12 results in the significant increase in the number of plasmid transformants. This phenomenon named "augmentation" was not observed when instead of intact cells, regenerating protoplasts were used, or if pBD12 DNA was introduced into the cells via transduction.

摘要

携带pUB110质粒的枯草芽孢杆菌细胞被同源质粒pBD12的DNA转化后,质粒转化子数量显著增加。当使用再生原生质体而非完整细胞时,或者通过转导将pBD12 DNA导入细胞时,未观察到这种名为“增强”的现象。

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