[枯草芽孢杆菌感受态细胞与原生质体中质粒转化的比较研究]
[Comparative study of plasmid transformation in competent cells and protoplasts of Bacillus subtilis].
作者信息
Lakomova N M, Glumova E F, Prozorov A A
出版信息
Genetika. 1982 Sep;18(9):1402-7.
PMID:6814983
Abstract
The effect of different factors which inhibit transformation with chromosomal DNA on plasmid transformation of competent cells and protoplasts was examined. The treatment with EDTA, dinitrophenol, CdCl2 and different temperature shifts affects significantly less plasmid transformation of protoplasts than plasmid and chromosomal transformation of competent cells. These data point out the different mechanisms of DNA uptake by competent cells and protoplasts.
摘要
研究了抑制染色体DNA转化的不同因素对感受态细胞和原生质体质粒转化的影响。用乙二胺四乙酸（EDTA）、二硝基苯酚、氯化镉（CdCl₂）处理以及不同温度变化对原生质体质粒转化的影响明显小于对感受态细胞质粒和染色体转化的影响。这些数据表明感受态细胞和原生质体摄取DNA的机制不同。
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