黑腹果蝇tRNA4Val的核苷酸序列。氯乙醛修饰辅助RNA测序。
The nucleotide sequence of tRNA4Val of Drosophila melanogaster. Chloroacetaldehyde modification as an aid to RNA sequencing.
作者信息
Addison W R, Gillam I C, Tener G M
出版信息
J Biol Chem. 1982 Jan 25;257(2):674-7.
PMID:6798031
Abstract
The nucleotide sequence of tRNA4Val from Drosophila melanogaster was determined to be pGUUUmCCGUm1GGUG psi AGCGGDU(acp3U)AUCACA psi CUGCCmUIACAm5CGCAGAAGm7GCCCCCGGT psi CGm1AUCCCGGGCGGAAACACCA. It is probable that residue C 49 is modified to m5C. The use of tRNA modified with chloroacetaldehyde to overcome secondary structure problems in sequencing is described.
摘要
黑腹果蝇tRNA4Val的核苷酸序列被确定为pGUUUmCCGUm1GGUGψAGCGGDU(acp3U)AUCACAψCUGCCmUIACAm5CGCAGAAGm7GCCCCCGGTψCGm1AUCCCGGGCGGAAACACCA。第49位残基C很可能被修饰为m5C。本文描述了使用经氯乙醛修饰的tRNA来克服测序中的二级结构问题。
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