Hayashi S, Gillam I C, Grigliatti T A, Tener G M
Chromosoma. 1982;86(2):279-92. doi: 10.1007/BF00288682.
Six purified tRNAs labeled with 125I by chemical or enzymatic methods were hybridized to polytene chromosomes of Drosophila melanogaster. The main chromosomal regions of hybridization wer: tRNAGly(GGA), 58A, 84C, and 90E; tRNALeu(2), 44E, 66B5-8, and 79F; tRNASer(2b), 86A, 88A9-12, and 94A6-8; tRNAThr(3), 47F and 87B; tRNAThr(4), 93A1-2; and tRNATyr(1 gamma), 19F, 22F-23A, 41, 50C1-4 and 85A. At 50C the hybridization of tRNATyr(1 gamma) was polymorphic in the giant strains. When the hybridization of three valine isoacceptors studied previously was re-investigated, it was found that only one hybridization site, 90BC, was shared between tRNAVal(3b) and tRNAVal(4). tRNAVal(3a) did not have any sites in common with the other two.
通过化学或酶促方法用¹²⁵I标记的六种纯化tRNA与黑腹果蝇的多线染色体杂交。杂交的主要染色体区域为：tRNAGly(GGA)，58A、84C和90E；tRNALeu(2)，44E、66B5 - 8和79F；tRNASer(2b)，86A、88A9 - 12和94A6 - 8；tRNAThr(3)，47F和87B；tRNAThr(4)，93A1 - 2；以及tRNATyr(1γ)，19F、22F - 23A、41、50C1 - 4和85A。在50C处，tRNATyr(1γ)在巨型品系中的杂交具有多态性。当重新研究先前研究的三种缬氨酸同工受体的杂交时，发现tRNAVal(3b)和tRNAVal(4)之间仅共享一个杂交位点90BC。tRNAVal(3a)与其他两者没有任何共同位点。
