Chan J C, Yang J A, Dunn M J, Agris P F, Wong T W
Nucleic Acids Res. 1982 Jun 25;10(12):3755-8. doi: 10.1093/nar/10.12.3755.
A glutamate tRNA from rat liver was purified. By means of post-labeling techniques, its nucleotide sequence was shown to be: pU-C-C-C-A-C-A-U-m1G-G-U-C-psi-A-G-C- G-G-D-D-A-G-G-A-U-U-C-C-U-G-G-psi-U-mcm5S2U-U-C-A-C-C-C-A-G-G-C-G- G-C-m5C-m5C-G-G-G-Tm-psi-C-G-A-C-U-C-C-C-G-G-U-G-U-G-G-G-A-A-C-C-AOH. The sequence is remarkably similar to that of tRNAGlu from Drosophila melanogaster. Only 10 out of 75 nucleotides in the two tRNAs are different.
从大鼠肝脏中纯化出一种谷氨酸转运RNA（tRNA）。通过后标记技术，其核苷酸序列显示为：pU-C-C-C-A-C-A-U-m1G-G-U-C-ψ-A-G-C-G-G-D-D-A-G-G-A-U-U-C-C-U-G-G-ψ-U-mcm5S2U-U-C-A-C-C-C-A-G-G-C-G-G-C-m5C-m5C-G-G-G-Tm-ψ-C-G-A-C-U-C-C-C-G-G-U-G-U-G-G-G-A-A-C-C-AOH。该序列与黑腹果蝇的tRNAGlu序列非常相似。两种tRNA的75个核苷酸中只有10个不同。
