Luporini P, Dallai R
J Cell Sci. 1981 Oct;51:1-13. doi: 10.1242/jcs.51.1.1.
In the ciliate Euplotes crassus, mixed cells of different compatible mating types pass through an induction period before agglutinating with each other by means of cilia in the mating reaction. We examined the ciliary membranes of cells involved in the mating reaction by the freeze-fracture technique and detected at least 5 distinct types of specialization, each indicated by a special congregation of intramembrane particles. Near to the necklace, at the ciliary base, we observed a set of several parallel transverse rows of 10-15 nm particles; a longitudinal row of 12 nm particles appeared more distally from the necklace, preferentially in replicas of intermediate regions of the ciliary shaft. These 3 specializations were common to both mating and vegetative cells. The other 2 appeared as more dynamic specializations found exclusively at least in their patterned organization, in the ciliary membranes of mating cells. Taking on the aspects of rosette-like arrays and patches, respectively, the former were positioned regularly on ciliary membrane bulges (containing an electron-opaque granule) and consisted of 8-9 nm particles; the latter had an elliptic shape and contained up to 50 closely packed 9-10 nm particles.
在纤毛虫类的粗壮真核草履虫中，不同兼容交配型的混合细胞在交配反应中通过纤毛相互凝集之前会经历一个诱导期。我们通过冷冻断裂技术检查了参与交配反应的细胞的纤毛膜，检测到至少5种不同类型的特化结构，每种结构都由膜内颗粒的特殊聚集表示。在项链状结构附近、纤毛基部，我们观察到一组由10 - 15纳米颗粒组成的几条平行横向排；一排12纳米的颗粒出现在离项链状结构更远的位置，优先出现在纤毛轴中间区域的复制品中。这3种特化结构在交配细胞和营养细胞中都很常见。另外2种特化结构表现为更具动态性的结构，至少在其模式化组织中仅在交配细胞的纤毛膜中发现。前者呈现出玫瑰花结状阵列的样子，位于纤毛膜凸起（含有一个电子不透明颗粒）上，由8 - 9纳米颗粒组成；后者呈椭圆形，包含多达50个紧密排列的9 - 10纳米颗粒。
