Pappenheimer A M, Harper A A, Moynihan M, Brockes J P
J Infect Dis. 1982 Jan;145(1):94-102. doi: 10.1093/infdis/145.1.94.
The effect of route of injection on the toxicity of intact diphtheria toxin, cross-reacting material (CRM45), and diphtherial fragment A was compared in several animal species. By ordinary routes of injection, neither CRM45 nor fragment A was toxic, even in species for which 0.1 micrograms of toxin/kg of body weight was lethal. After intracerebral injection, however, small amounts of CRM45 led to paralysis and death, even in mice and rats--species that are resistant to toxin administered intravenously. High doses of fragment A were nontoxic even by the intracerebral route. The cytotoxic dose od CRM45 was approximately 10(-7) M for a variety of cell lines derived from toxin-sensitive or toxin-resistant species. Cultured at Schwann's cells, however, were more sensitive CRM45 than other cell lines tested and 50-100 times more sensitive to toxin than cells cultured from other adult rat tissues. Fragment A has virtually no cytotoxicity for any mammalian cell line tested.
比较了注射途径对完整白喉毒素、交叉反应物质（CRM45）和白喉片段A在几种动物物种中的毒性影响。通过常规注射途径，CRM45和片段A均无毒性，即使在每千克体重注射0.1微克毒素会致死的物种中也是如此。然而，脑内注射后，即使在对静脉注射毒素有抗性的小鼠和大鼠中，少量的CRM45也会导致麻痹和死亡。即使通过脑内途径，高剂量的片段A也无毒。对于来自毒素敏感或毒素抗性物种的多种细胞系，CRM45的细胞毒性剂量约为10^(-7)M。然而，雪旺氏细胞培养物比其他测试细胞系对CRM45更敏感，并且比对其他成年大鼠组织培养的细胞对毒素的敏感性高50至100倍。片段A对任何测试的哺乳动物细胞系几乎都没有细胞毒性。
