噬菌体SPP1中转化DNA的分子起源

Molecular origin of transducing DNA in bacteriophage SPP1.

作者信息

de Lencastre H, Archer L J

出版信息

J Gen Microbiol. 1981 Feb;122(2):345-9. doi: 10.1099/00221287-122-2-345.

Abstract

Transducing particles produced by bacteriophage SPP1 infection of Bacillus subtilis were separated from plaque-forming units by CsCl density-gradient centrifugation. The density in CsCl of DNA isolated from such purified transducing particles was similar to that of bacterial DNA, indicating that the transducing particles probably contain DNA of exclusively bacterial origin. Bacterial DNA synthesized after infection of the donor culture was also encapsulated in the transducing particles. The number of transducing particles was at least 10 times higher than that of the transductants.

摘要

通过氯化铯密度梯度离心，将枯草芽孢杆菌被噬菌体SPP1感染后产生的转导颗粒与噬菌斑形成单位分离。从这种纯化的转导颗粒中分离出的DNA在氯化铯中的密度与细菌DNA相似，这表明转导颗粒可能仅含有细菌来源的DNA。供体培养物感染后合成的细菌DNA也被包裹在转导颗粒中。转导颗粒的数量至少比转导子的数量高10倍。