Lopes J D, Caulada Z, Barbieri C L, Camargo E P
Am J Trop Med Hyg. 1981 Nov;30(6):1183-8. doi: 10.4269/ajtmh.1981.30.1183.
Immunological cross-reactivity between Trypanosoma cruzi and insect trypanosomatids was demonstrated by immunofluorescence and confirmed by complement fixation, direct agglutination and cross-absorption experiments. As antigens, the following organisms were surveyed: Crithidia deanei, Crithidia fasciculata, Crithidia luciliae, Herpetomonas samuelpessoai, Herpetomonas megaseliae, Herpetomonas muscarum muscarum, Leptomonas seymouri and Blastocrithidia culicis. Sera from patients with Chagas' disease or sera from rabbits immunized against various trypanosomatids were used as sources of antibodies. The demonstration of cross-reactivity was followed by a survey of 500 human sera (from normal persons or Chagas' disease patients) by immunofluorescence using insect trypanosomatids (H. muscarum muscarum, C. fasciculata and L. seymouri) as antigens. With H. muscarum muscarum 98.7% coincident positive results and 100% of coincident negative results were obtained. These findings may validate the use of insect trypanosomatids as an alternative source of antigen in the serodiagnosis of Chagas' disease by indirect immunofluorescence.
克氏锥虫与昆虫锥虫之间的免疫交叉反应通过免疫荧光得以证实，并经补体结合、直接凝集和交叉吸收实验加以确认。作为抗原，对以下生物体进行了检测：德氏克氏锥虫、 fasciculata 克氏锥虫、 luciliae 克氏锥虫、 samuelpessoai 赫氏锥虫、 megaseliae 赫氏锥虫、 muscarum muscarum 赫氏锥虫、 seymouri 利什曼原虫和 culicis 巴氏克氏锥虫。来自恰加斯病患者的血清或针对各种锥虫免疫的兔血清用作抗体来源。在证实交叉反应后，使用昆虫锥虫（ muscarum muscarum 赫氏锥虫、 fasciculata 克氏锥虫和 seymouri 利什曼原虫）作为抗原，通过免疫荧光对500份人血清（来自正常人或恰加斯病患者）进行了检测。使用 muscarum muscarum 赫氏锥虫时，获得了98.7%的一致阳性结果和100%的一致阴性结果。这些发现可能验证了昆虫锥虫作为抗原的替代来源在恰加斯病间接免疫荧光血清诊断中的应用。
