使用非致病性锥虫抗原进行美洲锥虫病的血清学诊断。
Serodiagnosis of American trypanosomosis by using nonpathogenic trypanosomatid antigen.
作者信息
Monteón V M, Guzmán-Rojas L, Negrete-García C, Rosales-Encina J L, Lopez P A
机构信息
División Auxiliar de Diagnóstico y Tratamiento, Instituto Nacional de Cardiología Ignacio Chávez, Mexico D.F. , Mexico.
出版信息
J Clin Microbiol. 1997 Dec;35(12):3316-9. doi: 10.1128/jcm.35.12.3316-3319.1997.
Abstract
Crithidia luciliae, a nonpathogenic trypanosomatid, could provide a good alternative source of antigen for serodiagnosis of Chagas' disease. An enzyme-linked immunosorbent assay showed 100% sensitivity and 83% specificity when 91 human serum samples from Chagas' disease patients and 127 human serum samples from people suffering from toxoplasmosis (21 samples), leishmaniasis (32 samples), systemic rheumatic diseases (33 samples), and heart diseases (41 samples) were tested simultaneously with Trypanosoma cruzi and C. luciliae crude extracts. By Western blotting, an immunodominant band (30 kDa) was recognized by chagasic sera on the C. luciliae crude extract; specificity reached 97% with respect to this protein band. The carbohydrate moieties were not antigenic.
摘要
克氏锥虫是一种非致病性锥虫，可为恰加斯病的血清学诊断提供良好的替代抗原来源。当用克氏锥虫和克氏利什曼原虫粗提物同时检测91份恰加斯病患者的人血清样本以及127份来自弓形虫病患者（21份样本）、利什曼病患者（32份样本）、系统性风湿性疾病患者（33份样本）和心脏病患者（41份样本）的人血清样本时，酶联免疫吸附试验显示敏感性为100%，特异性为83%。通过蛋白质印迹法，恰加斯病血清在克氏利什曼原虫粗提物上识别出一条免疫显性带（30 kDa）；针对该蛋白带，特异性达到97%。碳水化合物部分没有抗原性。
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