亚油酸酶促氧化和非酶促氧化中氧同位素重排的研究。

A study of oxygen isotope scrambling in the enzymic and non-enzymic oxidation of linoleic acid.

作者信息

Schieberle P, Grosch W, Kexel H, Schmidt H L

出版信息

Biochim Biophys Acta. 1981 Dec 23;666(3):322-6. doi: 10.1016/0005-2760(81)90290-3.

Abstract

Lipoxygenases-1 and -2 isolated from soybeans were incubated with linoleic acid in the presence of a mixture of 16O2 and 18O2. The formation of 16O/18O-molecules which is indicative for a head-to head reaction of peroxy radicals was determined and compared with that produced during autoxidation of a linoleic acid emulsion in the presence of ferric ions. Lipoxygenase-1 was much less active in scrambling than lipoxygenase-2 which was comparable to that found in the autoxidation reaction.

摘要

从大豆中分离出的脂氧合酶-1和脂氧合酶-2在16O2和18O2的混合物存在下与亚油酸一起孵育。测定了16O/18O分子的形成情况，其表明过氧自由基的头对头反应，并与在铁离子存在下亚油酸乳液自氧化过程中产生的情况进行了比较。脂氧合酶-1在自由基重排方面的活性远低于脂氧合酶-2，脂氧合酶-2的活性与自氧化反应中的相当。