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果蝇中蜕皮激素诱导位点2B5处的转录

Transcription at the ecdysone-inducible locus 2B5 in Drosophila.

作者信息

Galcerán J, Llanos J, Sampedro J, Pongs O, Izquierdo M

机构信息

Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain.

出版信息

Nucleic Acids Res. 1990 Feb 11;18(3):539-45. doi: 10.1093/nar/18.3.539.

DOI:10.1093/nar/18.3.539
PMID:2106669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC333459/
Abstract

The Broad-Complex (BR-C) of D. melanogaster, mapping at the 2B5 early ecdysone puff, mediates ecdysone-induced processes. We present here the transcriptional analysis of the locus in both wild type and representative mutants. Two well defined regions are transcribed, only one of which has a pattern consistent with the proposed 2B5 located BR-C function. The 2B5 region is actively transcribed in early third instar larvae before ecdysone levels increase. Ecdysone switches this early pattern to a complex late type which requires the presence of functional BR-C product. Therefore, BR-C self-regulates its own ecdysone-induced transcription. The effect of 2B5 mutations on transcription at several intermolt, early and late puffs is also described. Null mutations at the 2B5 locus prevents ecdysone inducible transcription. This evidence supports the fact that 2B5 codes for an ecdysone-dependent transcriptional regulator.

摘要

黑腹果蝇的泛复合体（BR-C）定位于2B5早期蜕皮激素胀泡，介导蜕皮激素诱导的过程。我们在此展示了野生型和代表性突变体中该基因座的转录分析。转录出两个明确的区域，其中只有一个区域的模式与所提出的位于2B5的BR-C功能一致。在蜕皮激素水平升高之前，2B5区域在三龄幼虫早期就活跃转录。蜕皮激素将这种早期模式转变为一种复杂的晚期模式，这需要有功能性的BR-C产物存在。因此，BR-C自我调节其自身的蜕皮激素诱导转录。还描述了2B5突变对几个蜕皮间期、早期和晚期胀泡转录的影响。2B5基因座的无效突变阻止了蜕皮激素诱导的转录。这一证据支持了2B5编码一种蜕皮激素依赖性转录调节因子的事实。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/bc20ce7c9724/nar00187-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/bff7021517ed/nar00187-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/1d6dcef8b29d/nar00187-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/f32344716173/nar00187-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/bc20ce7c9724/nar00187-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/bff7021517ed/nar00187-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/1d6dcef8b29d/nar00187-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/f32344716173/nar00187-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a134/333459/bc20ce7c9724/nar00187-0142-a.jpg

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本文引用的文献

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Cytogenetic analysis of the 2B3-4--2B11 region of the X chromosome of Drosophila melanogaster. III. Puffing disturbance in salivary gland chromosomes of homozygotes for mutation l(1)pp1t10.黑腹果蝇X染色体2B3-4--2B11区域的细胞遗传学分析。III. 突变l(1)pp1t10纯合子唾液腺染色体中的胀泡干扰
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Staging the metamorphosis of Drosophila melanogaster.
一种独特的核糖核蛋白复合体优先组装在黑腹果蝇的蜕皮激素反应位点上。
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