Nakashima I, Takashi M, Nagase F, Kato N
Eur J Immunol. 1981 Nov;11(11):946-8. doi: 10.1002/eji.1830111118.
Intravenous injection of fluorescein isothiocyanate-modified syngeneic spleen cells (FITC-SSC) into mice induced T cell-independent, anti-hapten plaque-forming cell responses. In contrast to the high immunogenicity of FITC-coupled SSC or bone marrow cells, thymus, lymph node or peritoneal exudate cells were poorly immunogenic. Further analysis showed that FITC-syngeneic peripheral blood red cells were as immunogenic as FITC-SSC containing a similar number of red cells. The immunogenicity of the red cells was confirmed by treatment of FITC-SSC with 0.83% ammonium chloride before sensitization with FITC. This procedure almost totally abolished the immunogenicity regardless of the concentration of hapten with which they were sensitized. In addition, heat treatment or fixation with glutaraldehyde destroyed the immunogenicity of FITC-SSC.
向小鼠静脉注射异硫氰酸荧光素修饰的同基因脾细胞（FITC - SSC）可诱导非T细胞依赖性抗半抗原斑块形成细胞反应。与FITC偶联的脾细胞或骨髓细胞的高免疫原性相反，胸腺、淋巴结或腹腔渗出细胞的免疫原性较差。进一步分析表明，FITC - 同基因外周血红细胞与含有相似数量红细胞的FITC - SSC具有相同的免疫原性。在用FITC致敏前，用0.83%氯化铵处理FITC - SSC证实了红细胞的免疫原性。该程序几乎完全消除了免疫原性，无论与之致敏的半抗原浓度如何。此外，热处理或用戊二醛固定会破坏FITC - SSC的免疫原性。
