Ramos T, Möller E, Möller G
Eur J Immunol. 1980 Feb;10(2):100-4. doi: 10.1002/eji.1830100206.
Fluorescein (FITC)-haptenated mouse spleen cells are capable of inducing a B cell immune response characterized by the production of antibodies directed against hapten-altered self structures. The induction of this response is thymus-independent and strictly dependent on the hapten concentration used for labeling the cells. Pretreatment of mice with immunogenic, labeled spleen cells strongly suppressed the plaque-forming cell response to a subsequent challenge with FITC-labeled spleen cells, sheep (SRC) or horse (HRC) red cells labeled with the same hapten and native FITC-dextran. Mice primed with lightly haptenated (nonimmunogenic) cells 7 days before challenge were completely unresponsive to the immunogenic dose of labeled cells and displayed a significantly reduced response to FITC-SRC or FITC-HRC. However, the response to FITC-dextran was enhanced, as compared to unprimed animals. The concept of immunogenic vs. nonimmunogenic requirements of an antigen to induce unresponsiveness, and the specificity of the B cell clones affected by suppression is discussed.
荧光素（异硫氰酸荧光素，FITC）偶联半抗原的小鼠脾细胞能够诱导一种B细胞免疫反应，其特征是产生针对半抗原改变的自身结构的抗体。这种反应的诱导不依赖胸腺，且严格依赖于用于标记细胞的半抗原浓度。用具有免疫原性的标记脾细胞对小鼠进行预处理，会强烈抑制对随后用FITC标记的脾细胞、用相同半抗原标记的绵羊（SRC）或马（HRC）红细胞以及天然FITC - 葡聚糖进行攻击时的空斑形成细胞反应。在攻击前7天用轻度偶联半抗原（无免疫原性）的细胞进行致敏的小鼠，对免疫原性剂量的标记细胞完全无反应，并且对FITC - SRC或FITC - HRC的反应显著降低。然而，与未致敏动物相比，对FITC - 葡聚糖的反应增强。本文讨论了抗原诱导无反应性的免疫原性与非免疫原性要求的概念，以及受抑制影响的B细胞克隆的特异性。
